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Toxicity determination of pesticides
Published:
2022-10-31
1. Spray method: a spray method designed to simulate the actual control situation in the forest. In order to reduce the test error and make every tested insect receive the same amount of medicine as far as possible, spray is required to have pressure to make the size of the fog point consistent and spray even.
1. Spray method: a spray method designed to simulate the actual control situation in the forest. In order to reduce the test error and make every tested insect receive the same amount of medicine as far as possible, spray is required to have pressure to make the size of the fog point consistent and spray even.
2. Immersion method: Dip the tested insects in different concentrations of diluted liquid medicine, take them out immediately, put them on absorbent paper to absorb too much liquid medicine, transfer them into each group of containers, give them fresh leaves as feed, cover the containers with gauze or gauze mesh, feed them indoors, record the room temperature, and observe the poisoning deaths at different times.
3. Drug film method: spray the drug on the surface to form a uniform drug film (usually a petri dish with filter paper, or take out the needles after dipping them in the drug solution), and then let the tested pests on it, let them contact with the drug film for a period of time, and then move back to the normal container for feeding, or observe the poisoning death in the process of contacting the drug film.
4. Dropping method: use quantitative liquid medicine to drip on the back of the chest of the test insect, and then observe the poisoning death regularly. The advantages of this method are: the dose can be expressed by the amount of drug (commonly microgram) received per gram of worm, the amount of drug is easy to control, and the test error is small; The disadvantage is that it is time-consuming, and the solvent used for treatment, the location of the droplet and the size of the droplet can affect the toxicity. In adopting this Law, we should take these factors into account and try our best to reach agreement in order to obtain accurate results.
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